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human small intestine marathon ready cdna kit  (TaKaRa)


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    TaKaRa human small intestine marathon ready cdna kit
    Human Small Intestine Marathon Ready Cdna Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 40 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/marathon+ready+cdna+kit/Human+Small+Intestine+Marathon+-Ready+cDNA/pmc07363140-648-6-12
    Average 94 stars, based on 40 article reviews
    human small intestine marathon ready cdna kit - by Bioz Stars, 2026-09
    94/100 stars

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    Rapid Amplification of cDNA Ends:

    Article Title: A mosquito 2-Cys peroxiredoxin protects against nitrosative and oxidative stresses associated with malaria parasite infection
    Article Snippet: .. Additional 5′ and 3′ sequences were obtained using rapid amplification of cDNA ends using the Marathon-Ready cDNA kit (Clontech) and the gene-specific primers P1, 5′-GGACTTTACCTTCGTCTGCCCGACCG, and P2, 5′-CGATGATGAACAGGCCACGGAATGG. .. The PCR amplimers were subcloned into pCR 2.1-TOPO and transformed into Top10 cells (Invitrogen).

    Synthesized:

    Article Title: Interdependence of Platelet-Derived Growth Factor and Estrogen-Signaling Pathways in Inducing Neonatal Rat Testicular Gonocytes Proliferation
    Article Snippet: .. Briefly, probes were synthesized using a rat testis cDNA Library (Marathon-Ready cDNA kit; Advantage, Clontech, Mountain View, CA) using the following set of primers (Biosynthesis Inc., Lewisville, TX): for Raf1 : 491–509, 5′-GCAAAGGACTGTGGTCAAT-3′; 1524–1503, 5′-CCATGAACAGCAGGATATTAAC-3′; for Map2k1 : 173–192, 5′-AGAAGGTGGGAGAGTTGAAG-3′; 1087–1066, 5′-TCTTGATGAAAGCATGTACCAT-3′; for Mapk3 : 196–216, 5′-AAGACCAGAGTGGCTATCAAG-3′; 985–964, 5′-AGTACTGTTCCAGGTAAGGGTG-3′; and for Pik3 : 3593–3611, 5′-AGCCTACAACATTGTGAGG-3′; 4574–4556, 5′-GCAGAGCTGAATGTTAACC-3′; and PCR kits (Clontech). ..

    Article Title: Changes in MAPK pathway in neonatal and adult testis following fetal estrogen exposure and effects on rat testicular cells.
    Article Snippet: Concerns have been raised about the possible role of the phytoestrogen genistein and the xenoestrogen Bisphenol A (BPA) as endocrine disruptors.. In the present study, we examined the effects of fetal exposure to genistein and BPA on the Mitogen-activated protein kinase (MAPK) pathway and on testicular cell populations in neonatal and adult rat testes.. At postnatal day (PND) 3, genistein (0.1–10 mg/kg/day) and BPA (1–200 mg/kg/day) induced Raf1 and Erk1/2 mRNA and protein increases in testes, mainly in Sertoli cells.

    cDNA Library Assay:

    Article Title: Interdependence of Platelet-Derived Growth Factor and Estrogen-Signaling Pathways in Inducing Neonatal Rat Testicular Gonocytes Proliferation
    Article Snippet: .. Briefly, probes were synthesized using a rat testis cDNA Library (Marathon-Ready cDNA kit; Advantage, Clontech, Mountain View, CA) using the following set of primers (Biosynthesis Inc., Lewisville, TX): for Raf1 : 491–509, 5′-GCAAAGGACTGTGGTCAAT-3′; 1524–1503, 5′-CCATGAACAGCAGGATATTAAC-3′; for Map2k1 : 173–192, 5′-AGAAGGTGGGAGAGTTGAAG-3′; 1087–1066, 5′-TCTTGATGAAAGCATGTACCAT-3′; for Mapk3 : 196–216, 5′-AAGACCAGAGTGGCTATCAAG-3′; 985–964, 5′-AGTACTGTTCCAGGTAAGGGTG-3′; and for Pik3 : 3593–3611, 5′-AGCCTACAACATTGTGAGG-3′; 4574–4556, 5′-GCAGAGCTGAATGTTAACC-3′; and PCR kits (Clontech). ..

    Article Title: Changes in MAPK pathway in neonatal and adult testis following fetal estrogen exposure and effects on rat testicular cells.
    Article Snippet: Concerns have been raised about the possible role of the phytoestrogen genistein and the xenoestrogen Bisphenol A (BPA) as endocrine disruptors.. In the present study, we examined the effects of fetal exposure to genistein and BPA on the Mitogen-activated protein kinase (MAPK) pathway and on testicular cell populations in neonatal and adult rat testes.. At postnatal day (PND) 3, genistein (0.1–10 mg/kg/day) and BPA (1–200 mg/kg/day) induced Raf1 and Erk1/2 mRNA and protein increases in testes, mainly in Sertoli cells.

    Polymerase Chain Reaction:

    Article Title: Interdependence of Platelet-Derived Growth Factor and Estrogen-Signaling Pathways in Inducing Neonatal Rat Testicular Gonocytes Proliferation
    Article Snippet: .. Briefly, probes were synthesized using a rat testis cDNA Library (Marathon-Ready cDNA kit; Advantage, Clontech, Mountain View, CA) using the following set of primers (Biosynthesis Inc., Lewisville, TX): for Raf1 : 491–509, 5′-GCAAAGGACTGTGGTCAAT-3′; 1524–1503, 5′-CCATGAACAGCAGGATATTAAC-3′; for Map2k1 : 173–192, 5′-AGAAGGTGGGAGAGTTGAAG-3′; 1087–1066, 5′-TCTTGATGAAAGCATGTACCAT-3′; for Mapk3 : 196–216, 5′-AAGACCAGAGTGGCTATCAAG-3′; 985–964, 5′-AGTACTGTTCCAGGTAAGGGTG-3′; and for Pik3 : 3593–3611, 5′-AGCCTACAACATTGTGAGG-3′; 4574–4556, 5′-GCAGAGCTGAATGTTAACC-3′; and PCR kits (Clontech). ..

    Article Title: Changes in MAPK pathway in neonatal and adult testis following fetal estrogen exposure and effects on rat testicular cells.
    Article Snippet: Concerns have been raised about the possible role of the phytoestrogen genistein and the xenoestrogen Bisphenol A (BPA) as endocrine disruptors.. In the present study, we examined the effects of fetal exposure to genistein and BPA on the Mitogen-activated protein kinase (MAPK) pathway and on testicular cell populations in neonatal and adult rat testes.. At postnatal day (PND) 3, genistein (0.1–10 mg/kg/day) and BPA (1–200 mg/kg/day) induced Raf1 and Erk1/2 mRNA and protein increases in testes, mainly in Sertoli cells.



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    Representation of the genomic organization and splicing patterns of Ighμ in teleosts and catfish. Ighμ generally encodes a VH domain, four constant (Cμ 1 to Cμ 4 ) domains and two transmembrane domains. For full-length cDNA Ighμ transcripts in bighead catfish, North African catfish and their hybrid catfish, three major molecular techniques were performed (A) . Patterns of PCR amplification products, 5’ RACE PCR (B) , internal constant domain (C) , and 3’ RACE PCR (D) of Ighμ of bighead catfish, North African catfish and their hybrid catfish. A single gene copy (NCBI accession no. MZ559374) and six different Ighμ transcripts were identified in bighead catfish, and five and one were expressed as membrane (NCBI accession nos. MN934742, MN934743, MN934744, MN934745 and MN934746) and secreted forms (NCBI accession no. MN934747), respectively. Moreover, two membrane forms of Ighμ transcripts had novel splicing patterns in teleosts and vertebrates (highlighted in yellow) (NCBI accession nos. MN934745 and MN934746) (E) . A single gene copy (NCBI accession no. MZ559375) and three different Ighμ transcripts were identified in North African catfish; two and one were expressed as membrane (NCBI accession no. MN934748 and MN934749) and secreted forms (NCBI accession no. MN934750), respectively (F) . Two gene copies (NCBI accession nos. MZ559376 and MZ559377) and two Ighμ transcripts were identified in the hybrid catfish (NCBI accession nos. MN934751 and MN934752) (G) . Comparisons of cytogenetic localization of Ighμ gene loci in bighead catfish (H) , North African catfish (I) and their hybrid catfish (J) . All nucleotide sequences are illustrated in supplementary files and the NCBI database corresponding to the provided NCBI accession numbers.

    Journal: Frontiers in Immunology

    Article Title: Mystifying Molecular Structure, Expression and Repertoire Diversity of IgM Heavy Chain Genes ( Ighμ) in Clarias Catfish and Hybrids: Two Novel Transcripts in Vertebrates

    doi: 10.3389/fimmu.2022.884434

    Figure Lengend Snippet: Representation of the genomic organization and splicing patterns of Ighμ in teleosts and catfish. Ighμ generally encodes a VH domain, four constant (Cμ 1 to Cμ 4 ) domains and two transmembrane domains. For full-length cDNA Ighμ transcripts in bighead catfish, North African catfish and their hybrid catfish, three major molecular techniques were performed (A) . Patterns of PCR amplification products, 5’ RACE PCR (B) , internal constant domain (C) , and 3’ RACE PCR (D) of Ighμ of bighead catfish, North African catfish and their hybrid catfish. A single gene copy (NCBI accession no. MZ559374) and six different Ighμ transcripts were identified in bighead catfish, and five and one were expressed as membrane (NCBI accession nos. MN934742, MN934743, MN934744, MN934745 and MN934746) and secreted forms (NCBI accession no. MN934747), respectively. Moreover, two membrane forms of Ighμ transcripts had novel splicing patterns in teleosts and vertebrates (highlighted in yellow) (NCBI accession nos. MN934745 and MN934746) (E) . A single gene copy (NCBI accession no. MZ559375) and three different Ighμ transcripts were identified in North African catfish; two and one were expressed as membrane (NCBI accession no. MN934748 and MN934749) and secreted forms (NCBI accession no. MN934750), respectively (F) . Two gene copies (NCBI accession nos. MZ559376 and MZ559377) and two Ighμ transcripts were identified in the hybrid catfish (NCBI accession nos. MN934751 and MN934752) (G) . Comparisons of cytogenetic localization of Ighμ gene loci in bighead catfish (H) , North African catfish (I) and their hybrid catfish (J) . All nucleotide sequences are illustrated in supplementary files and the NCBI database corresponding to the provided NCBI accession numbers.

    Article Snippet: The 5′ VH region of first-strand cDNAs encoding Ighμ genes was synthesized from total RNA of PBLs of each catfish species using a 5′ RACE-Ready cDNA synthesis kit (Clontech, Mountain View, CA, USA), as previously described.

    Techniques: Amplification